Review

clp models surgery (MedChemExpress)

MedChemExpress is a verified supplier

MedChemExpress manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

MedChemExpress clp models surgery

Lack <t>of</t> <t>SIRT1</t> activity exacerbate sepsis-induced ALI and sepsis severity. (A) Histologic analysis of lung tissues (scale bars, 50 μm). (B) Lung injury score. (C) Representative blots. (D) SIRT1 expression in lung tissue. (E) Relative SIRT1 activity in lung tissue. (F) Histologic analysis of hearts, liver, and kidney tissues (scale bars, 50 μm). (G) Representative in situ detection of lung parenchymal cell apoptosis and endothelial cell apoptosis by TUNEL staining (red) and CD31 (green) (scale bars, 50 μm). (H) Percentage of TUNEL-positive nuclei. (I) Percentage of TUNEL-positive endothelial cells. (J) Lung MPO activity. (K) Survival after cecal ligation and puncture <t>(CLP)</t> ( n =12). (L) Rectal temperature. (M) Weight loss after CLP. n =8 in each group except for survival analysis. The data shown in (B)–(E), (I)–(J), and (L)–(M) represent means±SD. Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction in (B), (D)–(E), (H)–(J), and (L)–(M). The Kaplan–Meier method was used followed by a log-rank (Mantel-Cox) test in (K).

Clp Models Surgery, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/clp models surgery/product/MedChemExpress
Average 93 stars, based on 18 article reviews

clp models surgery - by Bioz Stars, 2026-06

93/100 stars

Images

1) Product Images from "SIRT1-Rab7 axis attenuates NLRP3 and STING activation through late endosomal-dependent mitophagy during sepsis-induced acute lung injury"

Article Title: SIRT1-Rab7 axis attenuates NLRP3 and STING activation through late endosomal-dependent mitophagy during sepsis-induced acute lung injury

Journal: International Journal of Surgery (London, England)

doi: 10.1097/JS9.0000000000001215

Lack of SIRT1 activity exacerbate sepsis-induced ALI and sepsis severity. (A) Histologic analysis of lung tissues (scale bars, 50 μm). (B) Lung injury score. (C) Representative blots. (D) SIRT1 expression in lung tissue. (E) Relative SIRT1 activity in lung tissue. (F) Histologic analysis of hearts, liver, and kidney tissues (scale bars, 50 μm). (G) Representative in situ detection of lung parenchymal cell apoptosis and endothelial cell apoptosis by TUNEL staining (red) and CD31 (green) (scale bars, 50 μm). (H) Percentage of TUNEL-positive nuclei. (I) Percentage of TUNEL-positive endothelial cells. (J) Lung MPO activity. (K) Survival after cecal ligation and puncture (CLP) ( n =12). (L) Rectal temperature. (M) Weight loss after CLP. n =8 in each group except for survival analysis. The data shown in (B)–(E), (I)–(J), and (L)–(M) represent means±SD. Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction in (B), (D)–(E), (H)–(J), and (L)–(M). The Kaplan–Meier method was used followed by a log-rank (Mantel-Cox) test in (K).

Figure Legend Snippet: Lack of SIRT1 activity exacerbate sepsis-induced ALI and sepsis severity. (A) Histologic analysis of lung tissues (scale bars, 50 μm). (B) Lung injury score. (C) Representative blots. (D) SIRT1 expression in lung tissue. (E) Relative SIRT1 activity in lung tissue. (F) Histologic analysis of hearts, liver, and kidney tissues (scale bars, 50 μm). (G) Representative in situ detection of lung parenchymal cell apoptosis and endothelial cell apoptosis by TUNEL staining (red) and CD31 (green) (scale bars, 50 μm). (H) Percentage of TUNEL-positive nuclei. (I) Percentage of TUNEL-positive endothelial cells. (J) Lung MPO activity. (K) Survival after cecal ligation and puncture (CLP) ( n =12). (L) Rectal temperature. (M) Weight loss after CLP. n =8 in each group except for survival analysis. The data shown in (B)–(E), (I)–(J), and (L)–(M) represent means±SD. Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction in (B), (D)–(E), (H)–(J), and (L)–(M). The Kaplan–Meier method was used followed by a log-rank (Mantel-Cox) test in (K).

Techniques Used: Activity Assay, Expressing, In Situ, TUNEL Assay, Staining, Ligation

Pharmacological inhibition of cGAS-STING and NLRP3 reduces lack of SIRT1 activity-induced lung endotheliopathy and sepsis severity in mice models with sepsis. (A) Representative blots. (B) pSTING expression. (C) GSDMD expression. (D) NLRP3 expression. (E) VCAM1 expression. (F) Histologic analysis of lung tissues (scale bars, 50 μm). (G) Lung injury score (medians, interquartile range). (H) lung MPO activity. (I) Evans blue dye leakage in ears ( n =5). (J) Quantitative analysis of the amount of extravasated Evans blue dye from mouse ears. §§ P <0.01 versus Con, ## P <0.01 versus CLP, ** P <0.01 versus CLP+EX527. n =8 in each group except for Evans blue dye leakage in ears. The data represent means±SD. Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction.

Figure Legend Snippet: Pharmacological inhibition of cGAS-STING and NLRP3 reduces lack of SIRT1 activity-induced lung endotheliopathy and sepsis severity in mice models with sepsis. (A) Representative blots. (B) pSTING expression. (C) GSDMD expression. (D) NLRP3 expression. (E) VCAM1 expression. (F) Histologic analysis of lung tissues (scale bars, 50 μm). (G) Lung injury score (medians, interquartile range). (H) lung MPO activity. (I) Evans blue dye leakage in ears ( n =5). (J) Quantitative analysis of the amount of extravasated Evans blue dye from mouse ears. §§ P <0.01 versus Con, ## P <0.01 versus CLP, ** P <0.01 versus CLP+EX527. n =8 in each group except for Evans blue dye leakage in ears. The data represent means±SD. Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction.

Techniques Used: Inhibition, Activity Assay, Expressing

Figure Legend Snippet: Pharmacological inhibition of cGAS-STING and NLRP3 reduces lack of SIRT1 activity-induced lung endotheliopathy and sepsis severity in mice models with sepsis. (A) Survival after cecal ligation and puncture (CLP) ( n =12). (B) Rectal temperature. (C) Weight loss after CLP. (D) Serum concentrations of IL-β. (E) Serum concentrations of IL-6. (F) Serum concentrations of TNF-α. ** P <0.01 versus CLP+EX527. n =8 in each group except for survival analysis. The data represent means±SD in (B)–(F). Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction in (B)–(F). The Kaplan–Meier method was used followed by a log-rank (Mantel-Cox) test in (A).

Techniques Used: Inhibition, Activity Assay, Ligation

Pharmacological restoration of SIRT1 activity ameliorated lung endotheliopathy and sepsis severity in mouse models of sepsis. (A) Representative blots. (B) pSTING expression. (C) GSDMD expression. (D) NLRP3 expression. (E) p62 expression. (F) VCAM1 expression. (G) Serum concentrations of Angpt2. (H) lung MPO activity. (I) Histologic analysis of lung tissues (scale bars, 50 μm). (J) Lung injury score. (K) Survival after cecal ligation and puncture (CLP) ( n =12). (L) Rectal temperature loss at 12 h. (M) Weight loss after CLP. # P <0.05 versus CLP, ## P <0.01 versus CLP, ** P <0.01 versus CLP+SRT1720. n =8 in each group except for survival analysis. The data represent means±SD in (B)–(H), (J), (L), and (M). Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction (A)–(J), (L), and (M). The Kaplan–Meier method was used followed by a log-rank (Mantel-Cox) test in (K).

Figure Legend Snippet: Pharmacological restoration of SIRT1 activity ameliorated lung endotheliopathy and sepsis severity in mouse models of sepsis. (A) Representative blots. (B) pSTING expression. (C) GSDMD expression. (D) NLRP3 expression. (E) p62 expression. (F) VCAM1 expression. (G) Serum concentrations of Angpt2. (H) lung MPO activity. (I) Histologic analysis of lung tissues (scale bars, 50 μm). (J) Lung injury score. (K) Survival after cecal ligation and puncture (CLP) ( n =12). (L) Rectal temperature loss at 12 h. (M) Weight loss after CLP. # P <0.05 versus CLP, ## P <0.01 versus CLP, ** P <0.01 versus CLP+SRT1720. n =8 in each group except for survival analysis. The data represent means±SD in (B)–(H), (J), (L), and (M). Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction (A)–(J), (L), and (M). The Kaplan–Meier method was used followed by a log-rank (Mantel-Cox) test in (K).

Techniques Used: Activity Assay, Expressing, Ligation

Pharmacological restoration of SIRT1 activity ameliorated lung endotheliopathy and sepsis severity in mouse models of sepsis. (A) Serum concentrations of angiopoietin 2 (Angpt2). (B) Representative blots. (C) VCAM1 expression. (D) Histologic analysis of lung tissues (scale bars, 50 μm). (E) Lung injury score. (F) Lung MPO activity. (G) Rectal temperature. (H) Weight loss after CLP. (I) Serum concentrations of IL-β. (J) Serum concentrations of IL-6. (K) Serum concentrations of TNF-α. n =5 in each group. The data represent means±SD. Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction.

Figure Legend Snippet: Pharmacological restoration of SIRT1 activity ameliorated lung endotheliopathy and sepsis severity in mouse models of sepsis. (A) Serum concentrations of angiopoietin 2 (Angpt2). (B) Representative blots. (C) VCAM1 expression. (D) Histologic analysis of lung tissues (scale bars, 50 μm). (E) Lung injury score. (F) Lung MPO activity. (G) Rectal temperature. (H) Weight loss after CLP. (I) Serum concentrations of IL-β. (J) Serum concentrations of IL-6. (K) Serum concentrations of TNF-α. n =5 in each group. The data represent means±SD. Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction.

Techniques Used: Activity Assay, Expressing

Image Search Results

Journal: International Journal of Surgery (London, England)

Article Title: SIRT1-Rab7 axis attenuates NLRP3 and STING activation through late endosomal-dependent mitophagy during sepsis-induced acute lung injury

doi: 10.1097/JS9.0000000000001215

Figure Lengend Snippet: Lack of SIRT1 activity exacerbate sepsis-induced ALI and sepsis severity. (A) Histologic analysis of lung tissues (scale bars, 50 μm). (B) Lung injury score. (C) Representative blots. (D) SIRT1 expression in lung tissue. (E) Relative SIRT1 activity in lung tissue. (F) Histologic analysis of hearts, liver, and kidney tissues (scale bars, 50 μm). (G) Representative in situ detection of lung parenchymal cell apoptosis and endothelial cell apoptosis by TUNEL staining (red) and CD31 (green) (scale bars, 50 μm). (H) Percentage of TUNEL-positive nuclei. (I) Percentage of TUNEL-positive endothelial cells. (J) Lung MPO activity. (K) Survival after cecal ligation and puncture (CLP) ( n =12). (L) Rectal temperature. (M) Weight loss after CLP. n =8 in each group except for survival analysis. The data shown in (B)–(E), (I)–(J), and (L)–(M) represent means±SD. Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction in (B), (D)–(E), (H)–(J), and (L)–(M). The Kaplan–Meier method was used followed by a log-rank (Mantel-Cox) test in (K).

Article Snippet: To understand the therapeutic potential of NLRP3 targeting in SIRT1 deficiency following CLP models surgery, MCC950 (50 mg/kg, MedChemExpress) were administered intraperitoneally at 1 h before the CLP surgery .

Techniques: Activity Assay, Expressing, In Situ, TUNEL Assay, Staining, Ligation

Journal: International Journal of Surgery (London, England)

Article Title: SIRT1-Rab7 axis attenuates NLRP3 and STING activation through late endosomal-dependent mitophagy during sepsis-induced acute lung injury

doi: 10.1097/JS9.0000000000001215

Figure Lengend Snippet: Pharmacological inhibition of cGAS-STING and NLRP3 reduces lack of SIRT1 activity-induced lung endotheliopathy and sepsis severity in mice models with sepsis. (A) Representative blots. (B) pSTING expression. (C) GSDMD expression. (D) NLRP3 expression. (E) VCAM1 expression. (F) Histologic analysis of lung tissues (scale bars, 50 μm). (G) Lung injury score (medians, interquartile range). (H) lung MPO activity. (I) Evans blue dye leakage in ears ( n =5). (J) Quantitative analysis of the amount of extravasated Evans blue dye from mouse ears. §§ P <0.01 versus Con, ## P <0.01 versus CLP, ** P <0.01 versus CLP+EX527. n =8 in each group except for Evans blue dye leakage in ears. The data represent means±SD. Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction.

Techniques: Inhibition, Activity Assay, Expressing

Journal: International Journal of Surgery (London, England)

Article Title: SIRT1-Rab7 axis attenuates NLRP3 and STING activation through late endosomal-dependent mitophagy during sepsis-induced acute lung injury

doi: 10.1097/JS9.0000000000001215

Figure Lengend Snippet: Pharmacological inhibition of cGAS-STING and NLRP3 reduces lack of SIRT1 activity-induced lung endotheliopathy and sepsis severity in mice models with sepsis. (A) Survival after cecal ligation and puncture (CLP) ( n =12). (B) Rectal temperature. (C) Weight loss after CLP. (D) Serum concentrations of IL-β. (E) Serum concentrations of IL-6. (F) Serum concentrations of TNF-α. ** P <0.01 versus CLP+EX527. n =8 in each group except for survival analysis. The data represent means±SD in (B)–(F). Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction in (B)–(F). The Kaplan–Meier method was used followed by a log-rank (Mantel-Cox) test in (A).

Techniques: Inhibition, Activity Assay, Ligation

Journal: International Journal of Surgery (London, England)

Article Title: SIRT1-Rab7 axis attenuates NLRP3 and STING activation through late endosomal-dependent mitophagy during sepsis-induced acute lung injury

doi: 10.1097/JS9.0000000000001215

Figure Lengend Snippet: Pharmacological restoration of SIRT1 activity ameliorated lung endotheliopathy and sepsis severity in mouse models of sepsis. (A) Representative blots. (B) pSTING expression. (C) GSDMD expression. (D) NLRP3 expression. (E) p62 expression. (F) VCAM1 expression. (G) Serum concentrations of Angpt2. (H) lung MPO activity. (I) Histologic analysis of lung tissues (scale bars, 50 μm). (J) Lung injury score. (K) Survival after cecal ligation and puncture (CLP) ( n =12). (L) Rectal temperature loss at 12 h. (M) Weight loss after CLP. # P <0.05 versus CLP, ## P <0.01 versus CLP, ** P <0.01 versus CLP+SRT1720. n =8 in each group except for survival analysis. The data represent means±SD in (B)–(H), (J), (L), and (M). Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction (A)–(J), (L), and (M). The Kaplan–Meier method was used followed by a log-rank (Mantel-Cox) test in (K).

Techniques: Activity Assay, Expressing, Ligation

Journal: International Journal of Surgery (London, England)

Article Title: SIRT1-Rab7 axis attenuates NLRP3 and STING activation through late endosomal-dependent mitophagy during sepsis-induced acute lung injury

doi: 10.1097/JS9.0000000000001215

Figure Lengend Snippet: Pharmacological restoration of SIRT1 activity ameliorated lung endotheliopathy and sepsis severity in mouse models of sepsis. (A) Serum concentrations of angiopoietin 2 (Angpt2). (B) Representative blots. (C) VCAM1 expression. (D) Histologic analysis of lung tissues (scale bars, 50 μm). (E) Lung injury score. (F) Lung MPO activity. (G) Rectal temperature. (H) Weight loss after CLP. (I) Serum concentrations of IL-β. (J) Serum concentrations of IL-6. (K) Serum concentrations of TNF-α. n =5 in each group. The data represent means±SD. Statistical significance was tested using one-way ANOVA with post-hoc Bonferroni correction.

Techniques: Activity Assay, Expressing

Review

Structured Review

Images

1) Product Images from "SIRT1-Rab7 axis attenuates NLRP3 and STING activation through late endosomal-dependent mitophagy during sepsis-induced acute lung injury"

Similar Products

Image Search Results